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withmacsima running buffer  (Miltenyi Biotec)


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    Miltenyi Biotec withmacsima running buffer
    Withmacsima Running Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/macsima+running+buffer/MACSima+Running+Buffer/10__1038_slash_s42003___026___11109___8-242-10-13
    Average 95 stars, based on 33 article reviews
    withmacsima running buffer - by Bioz Stars, 2026-11
    95/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: Single Cell Analysis Reveals the Presence of Novel Intermediate Cells in Both Mice and Patients With Severe MASLD
    Article Snippet: .. Liver frozen embedded tissues, 4 μm thick, were mounted on SuperFrost Plus slides and fixed with a 4% paraformaldehyde solution for 10 min at RT, rinsed three times with MACSima Running Buffer (130- 121- 565, Miltenyi Biotec, Bergisch Gladbach, Germany), and placed onto MACSwell Four Imaging Frames (130- 124- 676, Miltenyi Biotec). .. Sections were then pretreated with DAPI at a 1:5 ratio (130- 127- 574, Miltenyi Biotec) in the Running Buffer for 20 min, washed three times with Running Buffer, and finalized with a sample volume of 250 μL.

    Article Title: Single Cell Analysis Reveals the Presence of Novel Intermediate Cells in Both Mice and Patients With Severe MASLD
    Article Snippet: .. Liver frozen embedded tissues, 4 μm thick, were mounted on SuperFrost Plus slides and fixed with a 4% paraformaldehyde solution for 10 min at RT, rinsed three times with MACSima Running Buffer (130‐121‐565, Miltenyi Biotec, Bergisch Gladbach, Germany), and placed onto MACSwell Four Imaging Frames (130‐124‐676, Miltenyi Biotec). .. Sections were then pre‐treated with DAPI at a 1:5 ratio (130‐127‐574, Miltenyi Biotec) in the Running Buffer for 20 min, washed three times with Running Buffer, and finalized with a sample volume of 250 μL.

    Staining:

    Article Title: Intravitreal AAV vector delivery induces integrin-dependent ocular inflammation, complement activation, and antiviral and DNA damage responses
    Article Snippet: Fixed ocular globes were sectioned at 7μM, and slides were loaded onto the MACSima Imaging System four-well cassette (Miltenyi Biotech). .. Slides were subsequently stained with DAPI for 10 min and washed thrice with MACSima running buffer (Miltenyi Biotech). ..

    Evaporation:

    Article Title: The Role of C/EBP-Homologous Protein in Idiopathic Inflammatory Myopathies.
    Article Snippet: .. The antibodies labelled with fluorochrome, including CD206- APC (REAL518), CD68- PE (REA1306), CD31- FITC (REA1028), and Collagen type I- APC (REAL958) at a dilution of 1:50 were set up in a MACSwell Deepwell Plate (130- 126- 865, Miltenyi Biotec) using MACSima Running Buffer, and subsequently sealed with MACSwell Sealing Foil (130- 126- 866, Miltenyi Biotec) to avoid evaporation. .. The obtained image datasets were employed for the following image processing conducted in MACS iQ View Software Version 1.3.2 (Miltenyi Biotec).



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    <t>MACSima</t> spatial profiling reveals subtle changes in cellularity in Bb- infected skin and LNs for wt and oppA2tn -infected mice. (A) Representative images of wt - (upper row) and oppA2tn -infected (lower row) mouse skin at 4 wpi. Note increased CD68 staining (in red, co-localized with CD11b in blue) indicative of macrophages in column 4. Scale bars represent 1,000 μm (B) Representative images of wt - (upper row) and oppA2tn -infected (lower row) mouse LN at 5 dpi. Note increased overall B cell density and increased CD45R (in lilac) staining indicative of B cells in wt -infected mice. CD3 + CD4 - cells were considered as CD8 + T cells. Scale bars represent 500μm.
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    <t>MACSima</t> spatial profiling reveals subtle changes in cellularity in Bb- infected skin and LNs for wt and oppA2tn -infected mice. (A) Representative images of wt - (upper row) and oppA2tn -infected (lower row) mouse skin at 4 wpi. Note increased CD68 staining (in red, co-localized with CD11b in blue) indicative of macrophages in column 4. Scale bars represent 1,000 μm (B) Representative images of wt - (upper row) and oppA2tn -infected (lower row) mouse LN at 5 dpi. Note increased overall B cell density and increased CD45R (in lilac) staining indicative of B cells in wt -infected mice. CD3 + CD4 - cells were considered as CD8 + T cells. Scale bars represent 500μm.
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    Image Search Results


    MACSima spatial profiling reveals subtle changes in cellularity in Bb- infected skin and LNs for wt and oppA2tn -infected mice. (A) Representative images of wt - (upper row) and oppA2tn -infected (lower row) mouse skin at 4 wpi. Note increased CD68 staining (in red, co-localized with CD11b in blue) indicative of macrophages in column 4. Scale bars represent 1,000 μm (B) Representative images of wt - (upper row) and oppA2tn -infected (lower row) mouse LN at 5 dpi. Note increased overall B cell density and increased CD45R (in lilac) staining indicative of B cells in wt -infected mice. CD3 + CD4 - cells were considered as CD8 + T cells. Scale bars represent 500μm.

    Journal: Frontiers in Immunology

    Article Title: Characterizing the impact of intracutaneous dissemination on host responses during Borrelia burgdorferi infection

    doi: 10.3389/fimmu.2026.1850665

    Figure Lengend Snippet: MACSima spatial profiling reveals subtle changes in cellularity in Bb- infected skin and LNs for wt and oppA2tn -infected mice. (A) Representative images of wt - (upper row) and oppA2tn -infected (lower row) mouse skin at 4 wpi. Note increased CD68 staining (in red, co-localized with CD11b in blue) indicative of macrophages in column 4. Scale bars represent 1,000 μm (B) Representative images of wt - (upper row) and oppA2tn -infected (lower row) mouse LN at 5 dpi. Note increased overall B cell density and increased CD45R (in lilac) staining indicative of B cells in wt -infected mice. CD3 + CD4 - cells were considered as CD8 + T cells. Scale bars represent 500μm.

    Article Snippet: Slides were then washed with Dawn dish soap (Proctor and Gamble) and stored in 1X MACSima Running Buffer (Miltenyi Biotec).

    Techniques: Infection, Staining